Grant ID RR210034
Awarded On May 19, 2021
Title Recruitment of First-Time, Tenure-Track Faculty Members
Program CPRIT Scholar
Award Mechanism Recruitment of First-Time, Tenure-Track Faculty Members
Institution/Organization The University of Texas Southwestern Medical Center
Principal Investigator/Program Director Sihan Wu
Cancer Sites Brain and Other Nervous System, Lung and Bronchus
Contracted Amount $2,000,000
Summary of Goals and Objectives

Goal 1: Identify cancer cell lines that carry ecDNAs and generate genomic maps of ecDNAs in each line.


  1. Reveal the ecDNA landscape of 329 cancer cell lines in the Cancer Cell Line Encyclopedia (CCLE) database by re-analyzing whole-genome sequencing (WGS) data and generating ecDNA reference maps containing genomic coordinates and gene annotations.
  2. Independently test the presence of ecDNAs in cell lines predicted to contain them by WGS and computational analysis.
  3. Validate ecDNA architecture by two-color FISH and generate 3D chromatin topology data by Hi-C.

Goal 2: Characterize ecDNA replication.


  1. Characte...

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